期刊
INTERNATIONAL AGROPHYSICS
卷 29, 期 3, 页码 299-306出版社
POLISH ACAD SCIENCES, INST AGROPHYSICS
DOI: 10.1515/intag-2015-0035
关键词
Opuntia stricta Haw. peels; polysaccharide extraction; water-soluble polysaccharides; antioxidant activity
类别
资金
- Ministry of Higher Education and Scientific Research of Tunisia
Opuntia stricta Haw. is considered as one of the most common cactus plant growing in Tunisia. Extracting valuable compounds from its fruit peel, considered as by-product, is drawing more and more attention, making it on the verge of commercialization. Water-soluble polysaccharides were extracted from Opuntia stricta Haw. peels, and their chemical composition assessed using thin layer chromatography. The antioxidant activities of the extracted polysaccharides were assessed using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, total antioxidant activity and reducing power capacity. The extraction yield of water-soluble polysaccharides was 7.53 +/- 0.86%. The chemical composition revealed the presence of rhamnose, arabinose, glucose, mannose, galactose and galacturonic acid. The infra-red spectroscopic analysis showed a similar structure to that of Opuntia ficus-indica polysaccharide peels. Additionally, the extracted polysaccharides exhibited high antioxidant activities. In fact, the free radical scavenging activity (half inhibition concentration = 6.5 mg ml(-1) with 94.9% inhibition at 50 mg ml(-1)), the total antioxidant activity (100 mu g ascorbic acid equivalent at 50 mg polysaccharides) and the reducing power capacity (absorbance 700 nm = 0.7 at 50 mg ml(-1)), appeared to be interesting compared to natural and synthetic antioxidants. Therefore, water-soluble polysaccharides from Opuntia stricta Haw. fruit peels could be a natural alternative to replace synthetic antioxidants.
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