期刊
EPILEPSY RESEARCH
卷 141, 期 -, 页码 56-63出版社
ELSEVIER
DOI: 10.1016/j.eplepsyres.2018.02.008
关键词
Mesenchymal stem cell; Epilepsy; Cell therapy; Mossy fiber sprouting
资金
- JSPS KAKENHI [16K09993]
- Northern Advancement Center for Science & Technology of Hokkaido Japan [H26 T-1-37, H29 SL-2]
- Akiyama Life Science Foundation
- Morinaga Houshikai
- RR&D Service of the U.S. Department of Veterans Affairs [B7335R, B9260L]
- BLRD Service of the U.S. Department of Veterans Affairs [B7335R, B9260L]
- National Multiple Sclerosis Society [RG2135]
- Connecticut Stem Cell Research Program [12-SCB-Yale-05]
- Grants-in-Aid for Scientific Research [16K09993] Funding Source: KAKEN
Objective: Status epilepticus (SE) causes neuronal cell death, aberrant mossy fiber sprouting (MFS), and cognitive deteriorations. The present study tested the hypothesis that systemically infused mesenchymal stem cells (MSCs) reduce epileptogenesis by inhibiting neuronal cell death and suppressing aberrant MFS, leading to cognitive function preservation in a rat model of epilepsy. Methods: SE was induced using the lithium-pilocarpine injection model. The seizure frequency was scored using a video-monitoring system and the Morris water maze test was carried out to evaluate cognitive function. Comparisons were made between MSCs-and vehicle-infused rats. Immunohistochemical staining was performed to detect Green fluorescent protein (GFP) + MSCs and to quantify the number of GAD67 + and NeuN + neurons in the hippocampus. Manganese-enhanced magnetic resonance imaging (MEMRI) and Timm staining were also performed to assess the MFS. Results: MSC infusion inhibited epileptogenesis and preserved cognitive function after SE. The infused GFP + MSCs were accumulated in the hippocampus and were associated with the preservation of GAD67+ and NeuN + hippocampal neurons. Furthermore, the MSC infusion suppressed the aberrant MFS in the hippocampus as evidenced by MEMRI and Timm staining. Conclusions: This study demonstrated that the intravenous infusion of MSCs mitigated epileptogenesis, thus advancing MSCs as an effective approach for epilepsy in clinical practice.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据