期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 112, 期 -, 页码 29-34出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2018.02.001
关键词
Monooxygenase; Oxide; Whole-cell biocatalyst; Epoxidation; Asymmetric synthesis
资金
- National Natural Science Foundation of China [21572220, 21372216, 21708038]
- Key Laboratory of Environmental and Applied Microbiology of the Chinese Academy of Sciences [KLCAS-2016-08, KLCAS-2015-01]
Styrene monooxygenases (SMOs) are highly stereoselective enzymes that catalyze the formation of chiral epoxides as versatile building blocks. To expand the enzyme toolbox, two bacterial SMOs were identified from the genome of marine microbes Paraglaciecola agarilytica NO2 and Marinobacteriwn litorale DSM 23545, and heterologously expressed in Escherichia coli in soluble form. Both of the resulting whole-cell biocatalysts exhibited maximal activity at 30 degrees C and pH 8.0. They catalyzed the sulfoxidation reactions, and the epoxidation of both conjugated and unconjugated styrene derivatives with up to > 99%ee. MISMO displayed higher activity toward most substrates tested. Compared to an established SMO from Pseudomonas species (PsSMO), MISMO achieved 3.0-, 3.4- and 2.6-fold conversions for substrates styrene, cinnamyl alcohol and 4-vinyl-2, 3-dihydrobenzofuran, respectively.
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