Modeling the pH-Ammonia Toxicity Relationship for Hydra viridissima in Soft Waters with Low Ionic Concentrations

There are limited data concerning the toxicity of ammonia in fresh soft waters. Ammonia toxicity is largely dependent on pH and temperature. The US Environmental Protection Agency (USEPA) has derived equations to adjust species toxicity estimates based on changes in pH and temperature. It has been reported that the pH-ammonia toxicity relationship, derived by the USEPA, may differ in waters with low ionic concentrations because of the absence of potentially ameliorative ionic constituents. The present study aimed to assess the pH-ammonia toxicity relationship for the tropical green hydra, Hydra viridissima, across a range of pH values in a natural water with low ionic content. Ammonia toxicity to H. viridissima was assessed at a pH range between 6.0 and 8.5 and temperature 27.5 +/- 1 degrees C. Test solution pH was maintained using a pH buffer. The resulting median effect concentrations ranged from 9.62 (7.95-11.65) mg L-1 total ammonia nitrogen at pH 6.0 to 0.64 (0.50-0.81) mg L-1 total ammonia nitrogen at pH 7.9. The results indicated that increasing pH increased the sensitivity of H. viridissima to ammonia. The pH dependence equation derived by the USEPA accurately described the relationship between pH and ammonia toxicity for H. viridissima. However, when the model parameters for the generic pooled relationship were used, the fit was less accurate (r(2) = 0.66), indicating that the generic pooled pH-dependence equations may not be appropriate for use with this species. (C) 2017 SETAC