期刊
ENVIRONMENTAL RESEARCH
卷 164, 期 -, 页码 45-52出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.envres.2018.02.011
关键词
Neuroprogenitor cells; Proliferation, differentiation; Obesity; Perinatal exposures; Epigenetic
资金
- National Institute of Environmental Health Sciences [R21ES023112-01]
- National Center for Advancing Translational Sciences UCLA- CTSI [U11TR000124]
- LABioMed Bridge Funding [531230]
- National Institute on Minority Health and Health Disparities [5U54MD007598-06]
- Flora Foundation
- National Institute on Minority Health and Health Disparities [U54MD007598] Funding Source: NIH RePORTER
In utero exposure to the ubiquitous plasticizer, bisphenol A (BPA) is associated with offspring obesity. As food intake/appetite is one of the critical elements contributing to obesity, we determined the effects of in vivo maternal BPA and in vitro BPA exposure on newborn hypothalamic stem cells which form the arcuate nucleus appetite center. For in vivo studies, female rats received BPA prior to and during pregnancy via drinking water, and newborn offspring primary hypothalamic neuroprogenitor (NPCs) were obtained and cultured. For in vitro BPA exposure, primary hypothalamic NPCs from healthy newborns were utilized. In both cases, we studied the effects of BPA on NPC proliferation and differentiation, including putative signal and appetite factors. Maternal BPA increased hypothalamic NPC proliferation and differentiation in newborns, in conjunction with increased neuroproliferative (Hes1) and proneurogenic (Ngn3) protein expression. With NPC differentiation, BPA exposure increased appetite peptide and reduced satiety peptide expression. In vitro BPA-treated control NPCs showed results that were consistent with in vivo data (increase appetite vs satiety peptide expression) and further showed a shift towards neuronal versus glial fate as well as an increase in the epigenetic regulator lysine-specific histone demethylase1 (LSD1). These findings emphasize the vulnerability of stem-cell populations that are involved in life-long regulation of metabolic homeostasis to epigenetically-mediated endocrine disruption by BPA during early life.
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