期刊
ENVIRONMENTAL POLLUTION
卷 237, 期 -, 页码 377-387出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2018.02.035
关键词
Hepatic fatty acids metabolism; Grape seed proanthocyanidin extract; Lead; Transcriptional regulation; PPAR alpha signaling pathway
资金
- National Natural Science Foundation of China [31101868]
- Scientific Research Foundation for Excellent Returned Scholars of Heilongjiang Province
- Financial Assistance from Postdoctoral Scientific Research Developmental Fund of Heilongjiang Province [LBH-Q16013]
- University Nursing Program for Young Scholar with Creative Talents in Heilongjiang Province [UNPYSCT-2016012]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars of Heilongjiang Province [LC2017007]
- Academic Backbone Support Program [15XG17]
Lead, a pervasive environmental hazard worldwide, causes a wide range of physiological and biochemical destruction, including metabolic dysfunction. Grape seed proanthocyanidin extract (GSPE) is a natural production with potential metabolic regulation in liver. This study was performed to investigate the protective role of GSPE against lead-induced metabolic dysfunction in liver and elucidate the potential molecular mechanism of this event. Wistar rats received GSPE (200 mg/kg) daily with or without lead acetate (PbA, 0.5 g/L) exposure for 56 d. According to biochemical and histopathologic analysis, GSPE attenuated lead-induced metabolic dysfunction, oxidative stress, and liver dysfunction. Liver gene expression profiling was assessed by RNA sequencing and validated by qRT-PCR. Expression of some genes in peroxisome proliferator-activated receptor alpha (PPAR alpha) signaling pathway was significantly suppressed in PbA group and revived in PbA GSPE group, which was manifested by Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis and validated by western blot analysis. This study supports that dietary GSPE ameliorates lead-induced fatty acids metabolic disturbance in rat liver associated with PPAR alpha signaling pathway, and suggests that dietary GSPE may be a protector against lead-induced metabolic dysfunction and liver injury, providing a novel therapy to protect liver against lead exposure. (C) 2018 Elsevier Ltd. All rights reserved.
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