期刊
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
卷 147, 期 -, 页码 53-62出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2017.11.008
关键词
Cysteine protease; Salix matsudana; Salt stress; Antioxidant
资金
- Biotechnology Research Center, China Three Gorges University, Yichang, China [2016KBC05]
- National Natural Science Foundation of China [31370600, 31300508]
- Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding [2016C02056-1]
- National Nonprofit Institute Research Grant of CAF [RISF2014010, CAFYBB2014QB014]
Salix matsudana is a salt-tolerant arbor tree species. We are interested in understanding its mechanisms of salt-tolerance. Here, we isolated a full-length 1080-bp cDNA for a salt stress-responsive cysteine protease gene from S. matsudana, designated SmCP. The deduced 359-amino-acid SmCP protein was essentially identical to cysteine proteases of other plant species and was predicted to contain inhibitor-I29 and peptidase-C1A domains with three active catalytic sites found in eukaryotic cysteine proteases. Expression profile analysis revealed that SmCP expression is constitutive and overexpression in roots takes place under salt stress. Expression of SmCP in Escherichia coli cells led to enhanced salinity tolerance. In addition, transgenic Arabidopsis thaliana lines over expressing SmCP displayed enhanced tolerance to salt stress, with increased germination rates, antioxidant enzyme (superoxide dismutase) activity, chlorophyll content and ion flux in the root, and reduced malondialdehyde (MDA) levels and electric conductivity compared with control plants. These results indicate that SmCP likely plays an important role in salt tolerance in S. matsudana.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据