期刊
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
卷 151, 期 -, 页码 21-31出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envexpbot.2018.03.018
关键词
Cell wall; Gene expression; Hemicellulose; Iron; Nitrogen form; Remobilization; Rice; Transport
资金
- National Key Research and Development Program of China [2016YFD0200801, 2017YFD0300100]
- Science and Technology Planning Project in Zhejiang Province [2015C26005]
- Basic Research Foundation of National Commonweal Research Institute [2017RG004-2]
- Zhejiang Provincial Natural Science Foundation of China [LY18C130005, Q15C130006]
NH4+ and NO3- are two major nitrogen sources that differentially affect the uptake and transfer of other nutrients in rice (Oryza sativa), although their effects on Fe remobilization under Fe-starvation conditions are largely unknown. Here, by investigating the rice cultivar 'Kasalath' (Kas), which is tolerant of nutrient-deficient soil, we found that NH4+ negatively regulates the hemicellulose 1 content in root cell walls and stimulates the secretion of phenolic compounds from roots under -Fe conditions, thus facilitating the release of cell wall Fe and increasing soluble Fe levels in roots. NH4+ significantly increased the translocation of Fe from root to shoot by upregulating the expression of OsFRDL1 in roots and increasing the Fe content in the xylem. We further found that the higher putrescine content in rice roots under NH4+ conditions led to a higher nitric oxide (NO) content compared with NO3- conditions, and that NO is involved in the NH4+-stimulated cell wall Fe reutilization. The addition of the NO donor sodium nitroprusside significantly reduced the hemicellulose 1 content in cell walls and increased Fe release from cell walls, ultimately increasing the soluble Fe content in rice and alleviating chlorosis under -Fe conditions. Taken together, our findings demonstrate that NH4+ promotes Fe remobilization from the cell wall in rice.
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