期刊
EMBO REPORTS
卷 19, 期 9, 页码 -出版社
WILEY
DOI: 10.15252/embr.201845775
关键词
caveolae; cavin; coiled-coil; undecad repeat
资金
- National Health and Medical Research Council of Australia (NHMRC) [APP569542, APP1037320]
- Australian Research Council (ARC) [DP120101298]
- NHMRC [APP1058565, APP1061574]
- Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology
- ACRF
Caveolae are plasma membrane invaginations involved in transport, signalling and mechanical membrane sensing in metazoans. Their formation depends upon multiple interactions between membrane-embedded caveolins, lipids and cytosolic cavin proteins. Of the four cavin family members, only cavin1 is strictly required for caveola formation. Here, we demonstrate that an eleven residue (undecad) repeat sequence (UC1) exclusive to cavin1 is essential for caveolar localization and promotes membrane remodelling through binding to phosphatidylserine. In the notochord of mechanically stimulated zebrafish embryos, the UC1 domain is required for caveolar stability and resistance to membrane stress. The number of undecad repeats in the cavin1 UC1 domain varies throughout evolution, and we find that an increased number also correlates with increased caveolar stability. Lastly, we show that the cavin1 UC1 domain induces dramatic remodelling of the plasma membrane when grafted into cavin2 suggesting an important role in membrane sculpting. Overall, our work defines a novel conserved cavin1 modular domain that controls caveolar assembly and stability.
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