期刊
EMBO REPORTS
卷 19, 期 3, 页码 -出版社
WILEY
DOI: 10.15252/embr.201744867
关键词
fission yeast; sexual differentiation; TORC1; tRNA precursor
资金
- JSPS KAKENHI [15J40037, 16K18542, 15H04333]
- Biosignal Research Center, Kobe University [281009]
- Grants-in-Aid for Scientific Research [16K07445, 16K18542, 15H04333, 15J40037] Funding Source: KAKEN
Target of rapamycin (TOR) kinase controls cell growth and metabolism in response to nutrient availability. In the fission yeast Schizosaccharomyces pombe, TOR complex 1 (TORC1) promotes vegetative growth and inhibits sexual differentiation in the presence of ample nutrients. Here, we report the isolation and characterization of mutants with similar phenotypes as TORC1 mutants, in that they initiate sexual differentiation even in nutrient-rich conditions. In most mutants identified, TORC1 activity is downregulated and the mutated genes are involved in tRNA expression or modification. Expression of tRNA precursors decreases when cells undergo sexual differentiation. Furthermore, overexpression of tRNA precursors prevents TORC1 downregulation upon nitrogen starvation and represses the initiation of sexual differentiation. Based on these observations, we propose that tRNA precursors operate in the S. pombe TORC1 pathway to switch growth mode from vegetative to reproductive.
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