期刊
EMBO REPORTS
卷 19, 期 4, 页码 -出版社
WILEY
DOI: 10.15252/embr.201744807
关键词
BMP; CK1; FAM83G; PAWS1; Wnt
资金
- UK MRC Career Development Fellowship
- U.K. MRC Prize PhD studentship
- U.K. Medical Research Council [MC_UU_12016/3]
- DSTT (Merck-Serono)
- Francis Crick Institute
- Cancer Research UK [FC001157]
- UK Medical Research Council [FC001157]
- Wellcome Trust [FC001157]
- DSTT (Boehringer-Ingelheim)
- DSTT (GlaxoSmithKline)
- MRC [MC_U117597140, MC_UU_00018/6] Funding Source: UKRI
The BMP and Wnt signalling pathways determine axis specification during embryonic development. Our previous work has shown that PAWS1 (also known as FAM83G) interacts with SMAD1 and modulates BMP signalling. Here, surprisingly, we show that overexpression of PAWS1 in Xenopus embryos activates Wnt signalling and causes complete axis duplication. Consistent with these observations in Xenopus, Wnt signalling is diminished in U2OS osteosarcoma cells lacking PAWS1, while BMP signalling is unaffected. We show that PAWS1 interacts and co-localises with the isoform of casein kinase 1 (CK1), and that PAWS1 mutations incapable of binding CK1 fail both to activate Wnt signalling and to elicit axis duplication in Xenopus embryos.
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