期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 78, 期 -, 页码 233-235出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2014.10.504
关键词
Fluorescent dye; Reactive oxygen species; Reactive nitrogen species; Thiobarbituric acid; F2-isoprostane; Antioxidant; alpha-Tocopherol; Radical scavenging; Free radicals
资金
- British Heart Foundation [FS/10/59/28533, FS/13/66/30445, PG/13/1/29801, FS/15/6/31298] Funding Source: researchfish
- British Heart Foundation [PG/13/1/29801, FS/10/59/28533, FS/15/6/31298, FS/13/66/30445] Funding Source: Medline
Free radicals and oxidants are now implicated in physiological responses and in several diseases. Given the wide range of expertise of free radical researchers, application of the greater understanding of chemistry has not been uniformly applied to biological studies. We suggest that some widely used methodologies and terminologies hamper progress and need to be addressed. We make the case for abandonment and judicious use of several methods and terms and suggest practical and viable alternatives. These changes are suggested in four areas: use of fluorescent dyes to identify and quantify reactive species, methods for measurement of lipid peroxidation in complex biological systems, claims of antioxidants as radical scavengers, and use of the terms for reactive species. (C) 2014 Elsevier Inc. All rights reserved.
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