期刊
ELECTROANALYSIS
卷 30, 期 8, 页码 1783-1792出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.201700654
关键词
Monolayer graphene; Chemical vapor deposition; DNA-biosensor; Electrochemical impedance spectroscopy; Anodization
资金
- USDA [2014-67021-21589]
- U.S. NSF [1606181]
- Yazd University Research Council
- Directorate For Engineering [1606181] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys [1606181] Funding Source: National Science Foundation
We report the effect of electrochemical anodization on the properties of monolayer graphene as the main aim of this research and consequently using the resulting label-free impedimetric biosensor for DNA sequences detection. Monolayer graphene was grown by chemical vapor deposition (CVD) with methane as precursor on copper foil, transferred onto a glassy carbon electrode and electrochemically anodized. Raman spectroscopy and X-Ray photo electron spectroscopy revealed enhancement of defect density, roughness and formation of C-O-C, C-O-H and C=O functional groups after anodization. Amine-terminated poly T probe was linked covalently to the carboxylic groups of anodized graphene by the zero-length linker to fabricate the impedance-based DNA biosensor. The anodized graphene electrode demonstrated a superior performance for electrochemical impedance detection of DNA. The DNA biosensor showed a large linear dynamic range from 2.0x10(-18) to 1.0x10(-12)M with a limit of detection of 1.0x10(-18)M using electrochemical impedance spectroscopy (EIS) method. Equivalent circuit modeling shows that DNA hybridization is detected through a change in charge transfer resistance.
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