期刊
DATA IN BRIEF
卷 4, 期 -, 页码 146-151出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.dib.2015.04.019
关键词
Proteasome; Activity assay; Fluorogenic peptides; Proteasome-Glo
资金
- Belgian Program on Interuniversity Poles of Attraction
- Belgian State (Prime Minister's Office, Science Policy Programming)
- Fonds National de la Recherche Scientifique (Televie) [7454313F]
- Fonds National de la Recherche Scientifique (F.R.S.M.) [T.0022.14]
- Fondation contre le Cancer (nonprofit organization, Belgium)
- Fonds J. Maisin (Belgium)
- Fondation Salus Sanguinis (Belgium)
- Ludwig Institute for Cancer Research
- WELBIO
- Fonds National de la Recherche Scientifique (F.R.S.-FNRS, Belgium)
We recently developed a new assay to measure proteasome activity in vitro (CAPA for capture proteasome assay) [1] based on proteasome capture on an antibody -coated plate. When used with lysates originating from cells expressing either standard proteasome, immunoproteasome or intermediate proteasomes beta 5i or beta 1i-beta 5i, this assay allows the individual monitoring of the chymotrypsin-like, trypsin-like and caspase-like activities of the corresponding proteasome subtypes. The efficiency and specificity of four proteasome inhibitors were studied using the CAPA assay, demonstrating the potential of this assay for the development of subtype-specific proteasome inhibitors. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license.
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