期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 80, 期 -, 页码 59-66出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2014.12.012
关键词
Oxalate decarboxylase; Spin trapping; BMPO; Superoxide; Carbon dioxide radical
资金
- National Science Foundation [CHE-121440, CHE-0845450]
- National Institutes of Health [DK061666]
- RET supplement [MCB-1158000]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1213440] Funding Source: National Science Foundation
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0845450] Funding Source: National Science Foundation
This contribution describes the trapping of the hydroperoxyl radical at a pH of 4 during turnover of wild-type oxalate decarboxylase and its T165V mutant using the spin-trap BMPO. Radicals were detected and identified by a combination of EPR and mass spectrometry. Superoxide, or its conjugate acid, the hydroperoxyl radical, is expected as an intermediate in the decarboxylation and oxidation reactions of the oxalate monoanion, both of which are promoted by oxalate decarboxylase. Another intermediate, the carbon dioxide radical anion was also observed. The quantitative yields of superoxide trapping are similar in the wild type and the mutant while it is significantly different for the trapping of the carbon dioxide radical anion. This suggests that the two radicals are released from different sites of the protein. (C) 2014 Elsevier Inc. All rights reserved.
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