An assessment of assumptions and uncertainty in deuterium-based estimates of terrestrial subsidies to aquatic consumers

The deuterium ratio (H-2/H-1) in tissue is often used to estimate terrestrial subsidies to aquatic consumers because of strongly differentiated values between terrestrial and aquatic primary producers. However, quantitative deuterium-based analyses of terrestrial resource assimilation are highly dependent on several poorly defined assumptions. We explored the sensitivity of these estimates to assumptions regarding environmental water contributions to consumer deuterium content (omega) and algal photosynthetic hydrogen discrimination (epsilon(H)). We also tested whether C-13/C-12 and H-2/H-1-based estimates of terrestrial resource assimilation give similar outcomes. The average of the 12 experiments that have directly estimated proportional contributions of environmental water to consumer tissue H-2/H-1 was 0.27 +/- 0.11 (mean +/- SD), with similar values for invertebrates and fish. Conversely, of the 28 field studies that have used H-2/H-1 to characterize aquatic food webs, all but one assume a value that is less than our current best estimate, usually substantially less. A reanalysis of the raw data from four recent case studies indicates the calculated terrestrial contribution to aquatic consumers is extremely sensitive to this assumption. When the authors' original assumptions were used (i.e., omega = 0.16 +/- 0.05), the estimated proportional contribution of terrestrial resources to aquatic consumers (theta(T)) averaged 29 +/- 17%, and when omega = 0.27 was used the average estimated assimilation of allochthonous resources was approximate to 0.00. A compilation of published photosynthetic hydrogen discrimination values for microalgae averaged epsilon(H) = -150 +/- 27 parts per thousand (SD, n = 99), and a sensitivity analysis showed the outcomes of these calculations were also strongly influenced by uncertainty in epsilon(H). There was no statistical association between C-13/C-12 and H-2/H-1-based estimates of terrestrial subsidies (r = -0.12, n = 274). This analysis indicates that the assumptions in deuterium-based estimates of terrestrial resource assimilation are highly influential but poorly constrained; therefore, the impact of these assumptions on calculated outputs must be carefully assessed and thoroughly reported. Due to the highly uncertain assumptions inherent in deuterium-based analyses, we urge much more caution when using this approach to estimate terrestrial subsidies to consumers in aquatic ecosystems.