期刊
DYES AND PIGMENTS
卷 152, 期 -, 页码 155-160出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2018.01.050
关键词
pH; Ratiometric; NIR-fluorescent probe; Imaging; Live cells
资金
- National Key R&D Program of China [2017YFC0803504]
- National Natural Science Foundation of China [21705105, 21475080, 21575084]
A novel ratiometric emission MR-fluorescence probe N,N-dimethyl-4-((1E, 3E)-4-(3,3-dimethyl-3H-indol-2-yl) buta-1,3-dienyl)benzenamine, namely, DIDBA, is facilely synthesized on the basis of ethylene bridging of indole derivatives and 4-(dimethylamino) cinnamaldehyde. The probe exhibits a remarkable NIR ratiometric fluorescence emission (F-618nm/F-697nm) characteristic with pK(a) 4.5, and the linear response over the pH range of 3.5-5.2. DIDBA also displays favorable features include large Stokes shift of 200 nm under acidic conditions, high sensitivity, good selectivity, fine photostability and reversibility. The probe has low cytotoxicity and excellent cell membrane permeability, and is applied successfully to visualize intracellular pH fluctuations in live cells without influence of autofluorescence and native cellular species in biological systems.
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