期刊
BIOMEDICAL MATERIALS
卷 10, 期 4, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1748-6041/10/4/045008
关键词
bone morphogenetic protein-2 (BMP-2); platelet-derived growth factor (PDGF-BB); beta-tricalcium phosphate (beta-TCP); mesenchymal stem cells (MSCs); robocasting; bone repair
资金
- Ministry of Science and Technology [MAT2011-23819]
- Proyecto Motiva of ACIISI
- Beca CajaCanarias para posgraduados
The aim of this work was to study the bone repair induced by bone morphogenetic protein-2 (BMP-2), rat mesenchymal stem cells (rMSCs), and platelet-derived growth factor (PDGF-BB) incorporated in a macroporous beta-tricalcium phosphate (beta-TCP) system fabricated by robocasting, and to identify the most beneficial combination in a critical rat calvaria defect. BMP-2 was formulated in microspheres to provide a prolonged, local concentration, whereas PDGF-BB, which acts during the initial stage of defect repair, was incorporated in a thin layer of crosslinked alginate. Approximately 80% of PDGF-BB and 90% of BMP-2 were released into the defect during the first 2 d and 3 weeks, respectively. Histological analyses indicated a minor synergistic effect in the BMP-2-MSC groups. In contrast, significant antagonism was found with combined BMP-2 and PDGF-BB defect treatment. The high-grade repair induced by BMP-2 rules out any advantage from combining BMP-2 with PDGF-BB or MSCs, at least with this scaffold and defect model.
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