期刊
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
卷 92, 期 1, 页码 37-42出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.diagmicrobio.2018.04.006
关键词
Influenza; Virus; Diagnostics; Isothermal; Amplification; Portable
资金
- Research and Development team, Orion Diagnostica Oy
Isothermal nucleic acid amplification methods can potentially shorten the amount of time required to diagnose influenza. We developed and evaluated a novel isothermal nucleic acid amplification method, RT-SIBA to rapidly detect and differentiate between influenza A and B viruses in a single reaction tube. The performance of the RT-SIBA Influenza assay was compared with two established RT-PCR methods. The sensitivities of the RT-SIBA, RealStar RT-PCR, and CDC RT-PCR assays for the detection of influenza A and B viruses in the clinical specimens were 98.8%, 100%, and 89.3%, respectively. All three assays demonstrated a specificity of 100%. The average time to positive result was significantly shorter with the RT-SIBA Influenza assay (<20 min) than with the two RT-PCR assays (>90 min). The method can be run using battery-operated, portable devices with a small footprint and therefore has potential applications in both laboratory and near-patient settings. (C) 2018 Elsevier Inc. All rights reserved.
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