期刊
DEVELOPMENTAL CELL
卷 44, 期 2, 页码 165-+出版社
CELL PRESS
DOI: 10.1016/j.devcel.2017.12.004
关键词
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资金
- Jane Coffin Childs postdoctoral fellowship
- NIH [R01 DE016402, S10 OD021664]
- Department of Defense Breast Cancer Research Program [W81XWH-10-1-1023, W81XWH-13-1-0221]
- NIH Common Fund [DP2 HD080351-01]
- Chan-Zuckerberg Biohub Investigator Program
- NSF [MCB-1330864]
- UCSF Program in Breakthrough Biomedical Research
- UCSF Center for Cellular Construction [DBI-1548297]
- NSF Science and Technology Center
- Direct For Biological Sciences [1330864, 1548297] Funding Source: National Science Foundation
- Div Of Biological Infrastructure [1548297] Funding Source: National Science Foundation
- Div Of Molecular and Cellular Bioscience [1330864] Funding Source: National Science Foundation
Many tissues fold into complex shapes during development. Controlling this process in vitro would represent an important advance for tissue engineering. We use embryonic tissue explants, finite element modeling, and 3D cell-patterning techniques to show that mechanical compaction of the extracellular matrix during mesenchymal condensation is sufficient to drive tissue folding along programmed trajectories. The process requires cell contractility, generates strains at tissue interfaces, and causes patterns of collagen alignment around and between condensates. Aligned collagen fibers support elevated tensions that promote the folding of interfaces along paths that can be predicted by modeling. Wedemonstrate the robustness and versatility of this strategy for sculpting tissue interfaces by directing the morphogenesis of a variety of folded tissue forms from patterns of mesenchymal condensates. These studies provide insight into the active mechanical properties of the embryonic mesenchyme and establish engineering strategies for more robustly directing tissue morphogenesis ex vivo.
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