期刊
DEVELOPMENTAL BIOLOGY
卷 438, 期 2, 页码 111-123出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2018.04.003
关键词
Pigmentation; Insect; Melanin; Cell trafficking; Live imaging; Pattern boundary
资金
- European Union's Horizon research and innovation programme under the Marie Sklodowska-Curie grant [701691]
- Ludwig-Maximilians University of Munich
- Amgen Scholar program of the LMU
- Max Planck Society
- China Scholarship Council
- Marie Curie Actions (MSCA) [701691] Funding Source: Marie Curie Actions (MSCA)
Pigmentation is a diverse and ecologically relevant trait in insects. Pigment formation has been studied extensively at the genetic and biochemical levels. The temporality of pigment formation during animal development, however, is more elusive. Here, we examine this temporality, focusing on yellow, a gene involved in the formation of black melanin. We generated a protein-tagged yellow allele m the fruit fly Drosophila melanogaster, which allowed us to precisely describe Yellow expression pattern at the tissue and cellular levels throughout development. We found Yellow expressed in the pupal epidermis in patterns prefiguring black pigmentation. We also found Yellow expressed in a few central neurons from the second larval instar to adult stages, including a subset of neurons adjacent to the clock neurons marked by the gene Pdf. We then specifically examined the dynamics of Yellow expression domain and subcellular localization in relationship to pigment formation. In particular, we showed how a late step of re-internalization is regulated by the large low-density lipoprotein receptor-related protein Megalin. Finally we suggest a new function for Yellow in the establishment of sharp pigmentation pattern boundaries, whereby this protein may assume a structural role, anchoring pigment deposits or pigmentation enzymes in the cuticle.
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