期刊
DEVELOPMENT
卷 145, 期 5, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.153460
关键词
Wnt/BMP signaling; Articular cartilage; Immobilization; Joint development; Muscle contraction; Mechanoregulation; Mechanosensitivity
资金
- India-Ireland Co-operative Science Programme [DST/INT/IRE/P-1/11]
- Department of Biotechnology, Ministry of Science and Technology (India) [BT/PR11202/MED/32/46/2008, BT/PR14857/BRB/10/891/2010]
- Science and Engineering Research Board of the Department of Science and Technology, Ministry of Science and Technology (India) [EMR/2015/001519]
- Council of Scientific and Industrial Research (India)
- Ministry of Human Resource Development (India)
- Irish Research Council
- European Molecular Biology Organization short-term fellowship
Dynamic mechanical loading of synovial joints is necessary for normal joint development, as evidenced in certain clinical conditions, congenital disorders and animal models where dynamic muscle contractions are reduced or absent. Although the importance of mechanical forces on joint development is unequivocal, little is known about the molecular mechanisms involved. Here, using chick and mouse embryos, we observed that molecular changes in expression of multiple genes analyzed in the absence of mechanical stimulation are consistent across species. Our results suggest that abnormal joint development in immobilized embryos involves inappropriate regulation of Wnt and BMP signaling during definition of the emerging joint territories, i.e. reduced beta-catenin activation and concomitant upregulation of pSMAD1/5/8 signaling. Moreover, dynamic mechanical loading of the developing knee joint activates Smurf1 expression; our data suggest that Smurf1 insulates the joint region from pSMAD1/5/8 signaling and is essential for maintenance of joint progenitor cell fate.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据