期刊
DENTAL MATERIALS JOURNAL
卷 37, 期 4, 页码 575-581出版社
JAPANESE SOC DENTAL MATERIALS DEVICES
DOI: 10.4012/dmj.2017-305
关键词
Heparin; Calcium phosphate; alpha-Tricalcium phosphate; Critical size defects; Surface modification
资金
- JSPS KAKENHI [25463062, 16K11667]
- Japan Agency for Medical Research and Development [15ek0109138h001]
- Grants-in-Aid for Scientific Research [25463062, 16K11667] Funding Source: KAKEN
Heparin binds to and modulates various growth factors, potentially augmenting the bone-forming capability of biomaterials. Here, alpha-tricalcium phosphate (alpha-TCP) granules were modified with peptide containing the marine mussel-derived adhesive sequence, which reacts with alpha-TCP surface, and cationic sequence, which binds to heparin (alpha-Ph). alpha-Ph retained the alpha-TCP phase and intergranule spaces after the surface modification. The existence of heparin on alpha-Ph granules was confirmed using X-ray photoelectron spectroscopy. Granules of alpha-TCP and alpha-Ph were implanted into critical-size defects in rat calvaria for 4 weeks. Micro-computed tomography, histological evaluation, and Alcian blue staining revealed that alpha-Ph induced superior bone formation compared with alpha-TCP. Newly formed bone on alpha-Ph was preferentially in contact with the Alcian blue-stained surfaces of granules. These results suggested that heparinization enhanced the early osteogenic capacity of alpha-TCP, possibly by modulating the secretion of Alcian blue-stained extracellular matrixes.
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