Use of Carica papaya enzymes for enhancement of H2 production and degradation of glucose, protein, and lipids

Anaerobic batch experiments were carried out to examine the effect of supplementation of mixed culture bacteria with Carica papayaas enzymes for enhancement of hydrogen yield from degradation of glucose, protein, and lipids. The results showed that hydrogen yield (HY) based on protein and lipids degradation increased from 52.2 +/- 7.5 to 130.6 +/- 8.5 ml/g(protein), and from 43.0 +/- 5.3 to 64.8 +/- 3.1 ml/g(lipid) respectively with addition of Carica papaya as enzymes source. This corresponded to substrate degradation efficiency of 51.3 +/- 4.4% for protein and 33.7 +/- 2.6% for lipids. However, the hydrogen yield and degradation efficiency of glucose was slightly improved by addition of Carica papayaas enzymes. (C) 2015 The Authors. Published by Elsevier Ltd.