期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 42, 期 -, 页码 93-100出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2017.11.010
关键词
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资金
- National Science Foundation [CAREER 1253384]
- National Institutes of Health (NIGMS) [R01 GM095832]
- Eli Lilly and Company
- Agilent Technologies
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1253384] Funding Source: National Science Foundation
Rapidly characterizing the three-dimensional structures of proteins and the multimeric machines they form remains one of the great challenges facing modern biological and medical sciences. Ion mobility-mass spectrometry based techniques are playing an expanding role in characterizing these functional complexes, especially in drug discovery and development workflows. Despite this expansion, ion mobility-mass spectrometry faces many challenges, especially in the context of detecting small differences in protein tertiary structure that bear functional consequences. Collision induced unfolding is an ion mobility-mass spectrometry method that enables the rapid differentiation of subtly-different protein isoforms based on their unfolding patterns and stabilities. In this review, we summarize the modern implementation of such gas-phase unfolding experiments and provide an overview of recent developments in both methods and applications.
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