期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 51, 期 -, 页码 32-38出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2017.11.004
关键词
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资金
- European Commission [FP7-ICT-2013-10, 610730]
- BBSRC [EVO-ENGINE BB/P020615/1]
- FP7-KBBE [613745]
- H2020 Marie Sklodowska-Curie [642738]
- EPSRC-BBSRC [BB/M017982/1]
- Wellcome Trust U.K [WT102944]
- Biotechnology and Biological Sciences Research Council [BB/P020615/1] Funding Source: researchfish
- Wellcome Trust [102944/Z/13/Z] Funding Source: researchfish
- BBSRC [BB/M017982/1, BB/P020615/1] Funding Source: UKRI
- Wellcome Trust [102944/Z/13/Z] Funding Source: Wellcome Trust
Conventional in vivo directed evolution methods have primarily linked the biomolecule's activity to bacterial cell growth. Recent developments instead rely on the conditional growth of bacteriophages (phages), viruses that infect and replicate within bacteria. Here we review recent phage-based selection systems for in vivo directed evolution. These approaches have been applied to evolve a wide range of proteins including transcription factors, polymerases, proteases, DNA-binding proteins, and protein-protein interactions. Advances in this field expand the possible applications of protein and RNA engineering. This will ultimately result in new biomolecules with tailor-made properties, as well as giving us a better understanding of basic evolutionary processes.
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