期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 52, 期 -, 页码 32-41出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.copbio.2018.02.004
关键词
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资金
- Allen Distinguished Investigator Award from the Paul G. Allen Frontiers Group
- Thorek Memorial Foundation
- US National Institutes of Health (NIH) [R01DA036865, U01HG007900, R41GM119914, P30AR066527]
- NIH Director's New Innovator Award [DP2OD008586]
- NIH Biotechnology Training Grant [T32GM008555]
- NIH Predoctoral Fellowship [F31NS105419]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [U01HG007900] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [P30AR066527] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R41GM119914, T32GM008555] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R21NS103007, F31NS105419] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON DRUG ABUSE [R01DA036865] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP2OD008586] Funding Source: NIH RePORTER
Developments in CRISPR/Cas9-based technologies provide a new paradigm in functional screening of the genome. Conventional screening methods have focused on high throughput perturbations of the protein-coding genome with technologies such as RNAi. However, equivalent methods for perturbing the non-coding genome have not existed until recently. CRISPR-based screening of genomic DNA has enabled the study of both genes and non-coding gene regulatory elements. Here we review recent progress in assigning function to the non-coding genome using CRISPR-based genomic and epigenomic screens, and discuss the prospects of these technologies to transforming our understanding of genome structure and regulation.
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