期刊
CURRENT ISSUES IN MOLECULAR BIOLOGY
卷 27, 期 -, 页码 1-36出版社
HORIZON SCIENTIFIC PRESS
DOI: 10.21775/cimb.027.001
关键词
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资金
- Indian council of Agricultural Research for funding under NPTC project
- Department of Science and Technology Government of India for JC Bose National Fellowship
The history of DNA sequencing dates back to 1970s. During this period, the two first-generation nucleotide sequencing techniques were developed. Subsequently, Sanger's dideoxy method of sequencing gained popularity over Maxam and Gilbert's chemical method of sequencing. However, in the last decade, we have observed revolutionary changes in DNA sequencing technologies leading to the emergence of next-generation sequencing (NGS) techniques. NGS technologies have enhanced the throughput and speed of sequencing combined with bringing down the overall cost of the process over a time. The major applications of NGS technologies being genome sequencing and resequencing, transcriptomics, metagenomics in relation to plant-microbe interactions, exon and genome capturing, development of molecular markers and evolutionary studies. In this review, we present a broader picture of evolution of NGS tools, its various applications in crop plants, and future prospects of the technology for crop improvement.
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