期刊
CURRENT BIOLOGY
卷 28, 期 5, 页码 722-+出版社
CELL PRESS
DOI: 10.1016/j.cub.2018.01.050
关键词
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资金
- Research Foundation-Flanders (FWO) [G039815N, G009412N, G0.602.11.N.10, 1.5.091.11.N.00]
- Interuniversity Attraction Poles Programme-Belgian State-Belgian Science Policy (IUAP) [VI/33]
- Biotechnology and Biological Sciences Research Council (BBSRC)
- University of Antwerp [TTBOF/29267, DOCPRO4/28300, BOFNOI/23154]
- BBSRC [G19475]
- Engineering and Physical Sciences Research Council (EPSRC) [BB/D019613/1]
- BBSRC Professional Research Fellowship [BB/G023972/1]
- Royal Society-Wolfson Merit Award
- BBSRC Strategic Studentship [BS/S/H/2005/12044]
- Royal Society of London Research Grant Arabidopsis Root Hair Functional Genomics.''
- R&D program of Plasma Advanced Technology for Agriculture and Food (Plasma Farming)'' through the National Fusion Research Institute of Korea (NFRI) - government funds
- VIB International PhD program fellowship
- Hercules Foundation [AUHA 09/001]
- Biotechnology and Biological Sciences Research Council [BB/G023972/1, BB/M001806/1, G19475, BB/L026848/1, BB/M012212/1, BB/J009717/1, BB/H020314/1] Funding Source: researchfish
- BBSRC [BB/H020314/1, BB/D019613/1, BB/J009717/1, BB/G023972/1, BB/M001806/1, BB/L026848/1, BB/M012212/1] Funding Source: UKRI
Root hairs facilitate a plant's ability to acquire soil anchorage and nutrients. Root hair growth is regulated by the plant hormone auxin and dependent on localized synthesis, secretion, and modification of the root hair tip cell wall. However, the exact cell wall regulators in root hairs controlled by auxin have yet to be determined. In this study, we describe the characterization of ERULUS (ERU), an auxin-induced Arabidopsis receptor-like kinase, whose expression is directly regulated by ARF7 and ARF19 transcription factors. ERU belongs to the Catharanthus roseus RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) subfamily of putative cell wall sensor proteins. Imaging of a fluorescent fusion protein revealed that ERU is localized to the apical root hair plasma membrane. ERU regulates cell wall composition in root hairs and modulates pectin dynamics through negative control of pectin methylesterase (PME) activity. Mutant eru (-/-) root hairs accumulate de-esterified homogalacturonan and exhibit aberrant pectin Ca2+-binding site oscillations and increased PME activity. Up to 80% of the eru root hair phenotype is rescued by pharmacological supplementation with a PME-inhibiting catechin extract. ERU transcription is altered in specific cell wall-related root hair mutants, suggesting that it is a target for feedback regulation. Loss of ERU alters the phosphorylation status of FERONIA and H+-ATPases 1/2, regulators of apoplastic pH. Furthermore, H+-ATPases 1/2 and ERU are differentially phosphorylated in response to auxin. We conclude that ERULUS is a key auxin-controlled regulator of cell wall composition and pectin dynamics during root hair tip growth.
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