期刊
CONNECTIVE TISSUE RESEARCH
卷 59, 期 -, 页码 35-41出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/03008207.2017.1412432
关键词
Long noncoding RNA; mesenchymal stromal cells; osteoblast; RNA-Seq; gene expression; osteogenesis
资金
- NIH/NIAMS [R01AR039588]
- NIH/NIDCR [R37 DE012528]
Long noncoding RNAs (lncRNAs) have recently emerged as novel regulators of lineage commitment, differentiation, development, viability, and disease progression. Few studies have examined their role in osteogenesis; however, given their critical and wide-ranging roles in other tissues, lncRNAs are most likely vital regulators of osteogenesis. In this study, we extensively characterized lncRNA expression in mesenchymal cells during commitment and differentiation to the osteoblast lineage using a whole transcriptome sequencing approach (RNA-Seq). Using mouse primary mesenchymal stromal cells (mMSC), we identified 1438 annotated lncRNAs expressed during MSC differentiation, 462 of which are differentially expressed. We performed guilt-by-association analysis using lncRNA and mRNA expression profiles to identify lncRNAs influencing MSC commitment and differentiation. These findings open novel dimensions for exploring lncRNAs in regulating normal bone formation and in skeletal disorders.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据