期刊
FOOD MICROBIOLOGY
卷 51, 期 -, 页码 69-73出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2015.05.001
关键词
Escherichia coli O157; Bacteriophages; T5-like; T1-like; T4-like; O1-like
资金
- Alberta Livestock and Meat Agency [2011F007R]
- China Scholarship Council (MOE-AAFC-PhD Research Program)
Efficacy of four bacteriophages (phages) and a cocktail for biocontrol of Escherichia coli O157 was assessed on beef samples stored at 4, 22 and 37 degrees C. Samples (3 x 3 x 1 cm) were contaminated with E. coli O157 (104 CFU/cm2) and treated with single phages: T5-like (T5), T1-like (T1), T4-like (T4) and O1-like (O1), or a cocktail at two titers: multiplicity of infection (MOI) = 1000 and MOI = 10. In contrast to previous studies, use of virucidal solution prevented over-estimation of phage efficacy. Irrespective of temperature and MOIs, T5 was most (P < 0.001) and O1 least (P < 0.05) effective for biocontrol of E. coli O157, with relative efficacy of other phages temperature dependent. At 4 degrees C, T1 (P < 0.05) and cocktail (P < 0.001) were more effective than T4. In contrast, T4 was equally (P = 0.08, at 37 degrees C) or less effective (P = 0.003, at 22 degrees C) than T5. Phages were more effective (P < 0.001) against E. coli O157 at warmer temperatures and high MOI. As the beef supply chain includes hours of storage or transport at temperatures near 4 degrees C, this study demonstrates phages could significantly reduce E. coli O157 during this period. (C) 2015 Elsevier Ltd. All rights reserved.
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