期刊
PLANT SIGNALING & BEHAVIOR
卷 10, 期 10, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15592324.2015.1052923
关键词
DELLA; GAF1; gibberellin; GID1; proteolysis; signaling; SLY1; transcription factor; TOPLESS
资金
- Japan Society for the Promotion of Science (JSPS) [26440148, 15H04392]
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan [24116525, 24118004]
- Grants-in-Aid for Scientific Research [24116525, 15K18555, 24118004, 15H04392, 26440148, 24118001] Funding Source: KAKEN
Gibberellins (GAs) are important phytohormones for plant growth and development. DELLAs are members of the plant-specific GRAS protein family and act as repressors of GA signaling. DELLAs are rapidly degraded in the presence of GAs. GA-GID1-DELLA complexes are recognized and ubiquitinated by the SCFSLY complex. The sleepy1 (sly1) F-box mutant exhibits dwarfism and low-germination phenotypes due to high accumulation of DELLAs. Overexpression of GID1 in the sly1 mutant partially rescues these phenotypes without degradation of DELLAs suggesting that proteolysis independent regulation of DELLAs exists in GA signaling. But the molecular mechanisms of non-proteolytic regulation of DELLA are largely unknown. Recently we identified a DELLA binding transcription factor, GAI-ASSOCIATED FACTOR1 (GAF1). GAF1 also interacts with co-repressor TOPLESS RELATED (TPR) in nuclei. DELLAs and TPR act as coactivator and corepressor of GAF1, respectively. GAs converts the GAF1 complex from transcriptional activator to repressor via degradation of DELLAs. The overexpression of PAM, lacking of DELLAs binding region of GAF1, partially rescue dwarf phenotypes of GA deficient or GA insensitive mutant. In this study, we investigate the relationship between non-proteolytic regulation of DELLAs and GA signaling via DELLA-GAF1 complex using modified yeast two-hybrid system.
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