期刊
FOOD HYDROCOLLOIDS
卷 43, 期 -, 页码 31-40出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2014.04.028
关键词
Beta-carotene; Protein; Nano-particles; Antioxidant activity; Caco-2 cells
资金
- National 125 Program [2011BAD23B02, 2013AA1022207]
- NSFC [31171686]
- 111 Project [B07029]
- [NSF-Jiangsu-BK2012556]
- [PCSIRT0627]
- [JUSRP11422]
Beta-carotene (BC) was encapsulated by sodium caseinate (SC), whey protein isolate (WPI), and soybean protein isolate (SPI) by the homogenization-evaporation method forming nanoparticles of 78, 90 and 370 nm diameter. Indices of the chemical antioxidant assays, the reducing power, DPPH radical scavenging activity, and the hydroxyl radical scavenging activity (OH center dot) were improved by nanoencapsulation, compared to free BC. Caco-2 cells were treated with dichlorofluorescin diacetate (DCFH-DA) to determine the cellular antioxidant activity (CAA) of BC. CAA of SC, WPI, and SPI nanoparticles was higher (60%) compared to BC alone (45%). FTIR-ATR showed the presence of BC in the nanoparticle preparations. XRD and DSC analyses suggested that BC is amorphous in the nanoparticles. BC encapsulated by WPI had the most advantageous release properties. Release was low with pepsin but high with trypsin suggesting that WPI might be the best protein delivery vehicle to deliver BC to the intestine. (C) 2014 Elsevier Ltd. All rights reserved.
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