期刊
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
卷 218, 期 -, 页码 1-12出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpb.2017.12.016
关键词
Macrobrachium nipponense; Crustacean; Opsin; RNA interference; Ovary maturation
资金
- Central Public-interest Scientific Institution Basal Research Fund CAFS [2017JBFZ05]
- National Natural Science Foundation of China [31572617]
- Science & Technology Supporting Program of Jiangsu Province [BE2016308]
- Central Public interest Scientific Institution Basal Research Fund, CAFS [2016HYZ-D0402]
- China Agriculture Research System-48 [CARS-48]
- Fund of Independent Innovation of Agricultural Sciences of Jiangsu Province [CX (15)1012-4]
- Fund of Three Innovations Engineering of Jiangsu Province [D2015-16]
- Science and Technology Development Fund of Wuxi [CLE02N1514]
Opsins are photoreceptors with important roles in reproductive regulation in birds and fishes. In the present study, we identified an opsin gene from the eyes of the oriental river prawn Macrobrachium nipponense using expressed sequence tag analysis and rapid amplification of cDNA ends. The full-length transcript contained 1382 base pairs, encoding 375 amino acids. It was classified into the long-wavelength opsin group by phylogenetic analysis, and designated Mn-LW. Mn-LW expression demonstrated significant seasonal variation in somatic tissues from both male and female prawns, with the highest expression in the eyes, and expression also shown in the ovary. The expression profiles of Mn-LW in eyes and ovary were positively related to ovarian development. In situ hybridization showed that Mn-LW was present in retinular cells in the eye and oocytes in the ovary. Injection of Mn-LW dsRNA in vivo effectively down-regulated Mn-LW expression levels compared with control levels. Mn-LW dsRNA injection also significantly reduced vitellogenin (Vg) expression, indicating a close relationship between Mn-LW and Vg in ovarian development. These results suggest that Mn-LW may play an important role in Vg synthesis and accumulation during ovarian maturation in M. nipponense.
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