期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 161, 期 -, 页码 654-661出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.11.043
关键词
Peroxidase; Magnetic nanoparticles; Biocatalysts; Wastewater treatment
资金
- European Union for the project Mat4Treat (H2020-MSCA-RISE) [645551]
- Torino University for the project Microbusters [Torino_call2014_L2_126]
- CONICET [PIP: 12-2013-01-00236CO]
- UNLP [11/X679]
- Compagnia di San Paolo
Peroxidase immobilization onto magnetic supports is considered an innovative strategy for the development of technologies that involves enzymes in wastewater treatment. In this work, magnetic biocatalysts were prepared by immobilization of soybean peroxidase (SBP) onto different silica-coated superparamagnetic iron oxide nanoparticles. The obtained magnetic biocatalysts were tested for the degradation of malachite green (MG), a pollutant often found in industrial wastewaters and with significant drawbacks for the human and environmental health. A deep physicochemical characterization of the materials was performed by means of X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), High Resolution-Transmission Electron Microscope (HR-TEM) and magnetization measurements among others techniques. Results showed high immobilization yield of SBP onto nanomaterials with excellent properties for magnetic recoverability. A partial loss of activity with respect to free SBP was observed, compatible with the modification of the conformational structure of the enzyme after immobilization. The structural modification depended on the amount (and thickness) of silica present in the hybrid materials and the activity yield of 43% was obtained for the best biocatalyst. Thermal stability and reusability capacity were also evaluated. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据