期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 167, 期 -, 页码 93-103出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2018.03.050
关键词
Poly (L-lactic) acid; Polarization; Nanofibers; Corona poling; Neuronal differentiation and regeneration; Neural repair
资金
- Fundacao para a Ciencia e a Tecnologia (FCT)
- Fundo Europeu de Desenvolvimento Regional Portugal (FEDER)
- QREN-COMPETE Portugal [PTDC/SAU-NMC/111980/2009]
- Institute for Biomedicine [UID/BIM/04501/2013]
- CBC research unit [PEst-OE/SAU/UI0482/2011]
- Associate Laboratory CICECO [PEst-C/CTM/LA0011/2013]
- QOPNA research unit [PEst-C/QUI/UI0062/2013]
- FCT [FRH/BD/47331/2008]
Tissue engineering is evolving towards the production of smart platforms exhibiting stimulatory cues to guide tissue regeneration. This work explores the benefits of electrical polarization to produce more efficient neural tissue engineering platforms. Poly (L-lactic) acid (PLLA)-based scaffolds were prepared as solvent cast films and electrospun aligned nanofibers, and electrically polarized by an in-lab built corona poling device. The characterization of the platforms by thermally stimulated depolarization currents reveals a polarization of 60 x 10(-10)C cm(-2) that is stable on poled electrospun nanofibers for up to 6 months. Further in vitro studies using neuroblastoma cells reveals that platforms' polarization potentiates Retinoic Acid-induced neuronal differentiation. Additionally, in differentiating embryonic cortical neurons, poled aligned nanofibers further increased neurite outgrowth by 30% (+70 mu m) over non-poled aligned nanofibers, and by 50% (+100 mu m) over control conditions. Therefore, the synergy of topographical cues and electrical polarization of poled aligned nanofibers places them as promising biocompatible and bioactive platforms for neural tissue regeneration. Given their long lasting induced polarization, these PLLA poled nanofibrous scaffolds can be envisaged as therapeutic devices of long shelf life for neural repair applications. (C) 2018 Elsevier B.V. All rights reserved.
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