期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 161, 期 -, 页码 147-155出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2017.10.045
关键词
U937 cell line; Asbestiform fibers; EPR; TEM; Carcinogenicity; Solid/cell interface
资金
- University of Urbino Carlo Bo (DiSPeA research grant)
Human monocyte U937 cell line was used as a model to verify the toxicity of erionite and offretite asbestiform zeolite fibers. As a presumed non-toxic reference, a fibrous scolecite zeolite was also used. To analyze the process of fiber ingestion into cells and the cells-fibers interactions, a spin-probe electron paramagnetic resonance (EPR) analysis was performed supported by transmission electron microscopy (TEM) and cell viability measurements as a function of the incubation time. Erionite fibers were fast internalized in the membrane mainly as aggregates with radical-solution drops trapped inside, and were found in the cytosol and at the nucleus. In 24 h, first erionite fibers rich in sodium and potassium, and then calcium-rich erionite fibers, induced cell necrosis. The offretite fibers formed rounding electron-dense filaments which transformed in curved filaments, initially perturbing the cell structure and interacting at the external surface more than erionite fibers. Such interactions probably diminished the toxic effect of offretite on cells. Interestingly, the presumed non-toxic scolecite fibers were partially internalized, inducing formation of swollen mitochondria and squared cells. Overall, the toxic effect of the fibrous zeolites was related to fiber morphology, chemical distribution of sites, structural variations and formation of aggregates. (C) 2017 Elsevier B.V. All rights reserved.
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