期刊
FOOD CONTROL
卷 57, 期 -, 页码 54-61出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2015.03.035
关键词
Flavonoid glycoside; Marker; Bee pollen; LC-MS/MS; Classification
资金
- National Natural Science Foundation of China [31201859]
- National Project of Risk Assessment for Quality and Safety of Special Agro-products
- Agricultural Science and Technology Innovation Program [CAAS-ASTIP-2015-IAR]
- National Key Project for Agro-product Quality & Safety Risk Assessment, PRC [GJFP2015010]
The aim of this study was to develop and validate a method for reliable discrimination of bee pollen from different floral origins via simultaneous analysis of three flavonoid glycosides. A simple sample preparation procedure consisted of an ultrasonic-assisted extraction followed by high performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) technique to effectively identify and differentiate ten kinds of bee pollens has been developed and optimized in this study. For sample extraction, several parameters were optimized including extraction solvents, extraction patterns, solvent volume, extraction duration and temperature. The analytical method was validated in terms of linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, matrix effect and carryover test using quality control sample. This method can consistently distinguish bee pollen by analyzing three flavonoid glycosides (quercetin-3-O-beta-D-glucosyl-(2 -> I)-beta-glucoside, kaempferol-3, 4'-di-O-beta-D-glucoside and kaempferol-3-O-beta-D-glucosyl-(2 -> I)-beta-D-glucoside). Therefore, this technique will be a suitable to be used as a discrimination method to identify the floral origin of bee pollen via the proposed marker components. (C) 2015 Elsevier Ltd. All rights reserved.
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