期刊
FOOD CHEMISTRY
卷 174, 期 -, 页码 538-546出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.11.090
关键词
Dieckol; Hemeoxygenase-1; NAD(P)H:quinine oxidoreductase 1; Glutathione S-transferase; Nrf2; MAPK
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2013R1A1A2009588]
- National Research Foundation of Korea [2013R1A1A2009588] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Dieckol was previously reported to exhibit antioxidant and anticancer activities in vitro studies. In this study, we characterised the mechanism underlying the dieckol-mediated expression of antioxidant and detoxifying enzymes. Dieckol suppressed the production of intracellular reactive oxygen species in the presence or absence of H2O2 and increased glutathione level in HepG2 cells. Dieckol enhanced the activities of antioxidant enzymes, and the expression of detoxifying enzymes including heme oxygenase-1 (HO-1), NAD(P)H:quinine oxidoreductase 1 (NQO1), and glutathione S-transferase (GST) in HepG2 cells. Enhanced expression of antioxidant and detoxifying enzymes by dieckol was presumed to be the activation of the nuclear factor erythroid-derived 2-like 2 (Nrf2) demonstrated by its nuclear translocation and transcriptional activity via activation of mitogen-activated protein kinases in HepG2 cells. Furthermore, we demonstrated dieckol induced the expression of HO-1 in mouse liver. These results demonstrate that the dieckol-mediated cytoprotection in HepG2 cells is mediated through a ROS-independent up-regulation of antioxidant and detoxifying enzymes via Nrf2 activation as well as its intrinsic antioxidant activity, suggesting that dieckol may be used as a natural cytoprotective agent. (C) 2014 Elsevier Ltd. All rights reserved.
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