期刊
ALEXANDRIA JOURNAL OF MEDICINE
卷 51, 期 1, 页码 19-23出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ajme.2014.03.004
关键词
Flame atomic absorption spectrophotometer; Zinc; Insulin; Method development; Method validation
A sensitive, reliable and relative fast method has been developed for the determination of total zinc in insulin by atomic absorption spectrophotometer. This designed study was used to optimize the procedures for the existing methods. Spectrograms of both standard and sample solutions of zinc were recorded by measuring the absorbance at 213.9 nm for determination of total zinc. System suitability parameters were evaluated and were found to be within the limits. Linearity was evaluated through graphical representation of concentration versus absorbance. Repeatability (intra-day) and intermediate precision (inter-day) were assessed by analyzing working standard solutions. Accuracy and robustness were experimented from the standard procedures. The percentage recovery of zinc was found to be 99.8%, relative standard deviation RSD 1.13%, linearity of determination LOD 0.0032 mu g/mL, and limit of quantization LOQ 0.0120 mu g/mL. This developed and proposed method was then validated in terms of accuracy, precision, linearity and robustness which can be successfully used for the quantization of zinc in insulin. (C) 2014 Alexandria University Faculty of Medicine. Production and hosting by Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据