期刊
FOOD CHEMISTRY
卷 177, 期 -, 页码 97-101出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.01.007
关键词
Anti-idiotypic antibody; Citrinin; Single-domain antibodies; Phage display; Phage ELISA
资金
- National Basic Research Program of China [2013CB127804]
- National Natural Science Foundation of China [NSFC-31360386, NSFC-31201360, NSFC-31171696]
- Program of State Key Laboratory of Food Science and Technology [SKLF-ZZA-201302]
Anti-idiotypic antibodies (Aids) can mimic antigen molecules and can thus offer an alternative to conventional antigens in immunoassays. In this study, citrinin (CIT) was chosen as a target analyte, and an anti-idiotypic single-domain antibody (VHH) was selected from a naive alpaca VHHs library to serve as a surrogate for CIT hapten. The phage-displayed VHH was used as a signal-amplification carrier to develop an indirect competitive phage enzyme-linked immunosorbent assay (P-ELISA) for the sensitive detection of CIT. The half-inhibition concentration (IC50) of P-ELISA was 10.9 mu g/kg, which was 9-fold better than that of conventional ELISA (IC50 = 102.1 mu g/kg). Results on P-ELISA analysis of naturally contaminated samples were also consistent with those obtained by conventional ELISA. In conclusion, the proposed P-ELISA demonstrates the potential use of phage-displayed anti-idiotypic VHH as surrogate for small molecules and signal-amplification carrier to improve assay performance for more sensitive analyte detection in food safety monitoring. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据