期刊
FOOD CHEMISTRY
卷 174, 期 -, 页码 348-355出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.11.051
关键词
Capillary electrophoresis-electrospray ionisation-mass spectrometry; Centrifugal ultrafiltration; Human milk; Nucleotide monophosphates; Processing; Freezing time
资金
- Ministerio de Ciencia e Innovacion, Spain [CTQ 2011-24075]
- Ministerio de Educacion, Spain (FPU grant)
A simple, efficient and green analytical method for the determination of free nucleotide monophosphates in human milk is proposed. It involves centrifugal ultrafiltration (CUF) as sample treatment and capillary electrophoresis-electrospray mass spectrometry (CE-ESI-MS) for separation and simultaneous quantification. The optimised method, applied to the analysis of human milk samples, included their dilution (1:5) with water followed by CUF treatment. No matrix effects were found. The method provided limits of detection between 0.08 and 0.13 mu g mL(-1) and limits of quantification between 0.26 and 0.431 mu g mL(-1). The intralaboratory repeatability and reproducibility afforded relative standard deviation values lower than 10%. The method was applied to the study of the effects of Holder pasteurisation and high-pressure processing on the nucleotide contents in samples from a human milk bank. The results showed concentration values between 0.5 and 10 mu g mL(-1), with higher concentrations for the samples treated by pasteurisation. The effect of freezing time on the content of nucleotides was also assessed. (C) 2014 Elsevier Ltd. All rights reserved.
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