期刊
FOOD CHEMISTRY
卷 188, 期 -, 页码 527-536出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2015.05.037
关键词
Microwave assisted extraction; HPLC-DAD-ESI-MS/MS; Phenolic compounds; Eclipta prostrata L.; Green analytical chemistry; Antioxidant activity
资金
- Ministry of Industry and Information Technology of China [20101109]
- National Natural Science Foundation of China [81274012, 81102820]
A simple and rapid method was developed using microwave-assisted extraction (MAE) combined with HPLC-DAD-ESI-MS/MS for the simultaneous extraction, identification, and quantification of phenolic compounds in Eclipta prostrata, a common herb and vegetable in China. The optimized parameters of MAE were: employing 50% ethanol as solvent, microwave power 400 W, temperature 70 degrees C, ratio of liquid/solid 30 mL/g and extraction time 2 min. Compared to conventional extraction methods, the optimized MAE can avoid the degradation of the phenolic compounds and simultaneously obtained the highest yields of all components faster with less consumption of solvent and energy. Six phenolic acids, six flavonoid glycosides and one coumarin were firstly identified. The phenolic compounds were quantified by HPLC-DAD with good linearity, precision, and accuracy. The extract obtained by MAE showed significant antioxidant activity. The proposed method provides a valuable and green analytical methodology for the investigation of phenolic components in natural plants. (C) 2015 Elsevier Ltd. All rights reserved.
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