Scientific validation of Siddha formulation Sirupeelai Samoola Kudineer in the treatment of renal calculi in zinc implantation model

Sirupeelai samoola kudineer prescribed for the treatment of urolithiasis was prepared as per Siddha text and validated in male Sprague Dawley rats using Zinc implantation model. Urolithiasis was induced by implantation of pre weighed and sterile zinc disc in the urinary bladder of the rats with supplementation of ethylene glycol for 3 weeks. After induction, rats were treated with SK (4.5 ml/kg) and at the end of the study, animals were scarified and zinc disc was removed from the urinary bladder. The effect of SK on disc weight, urinary volume, pH and specific gravity besides qualitative analysis of, urinary parameters was investigated at 0th, 7th, 14th and 21st day. SK treatment reduced weight of the stones significantly when compared to its vehicle control. A significant decrease in the levels of stone and renal markers was observed in the SK treated group when compared to vehicle control group. A significant increase in the levels of antioxidants such as GSH, GPx, SOD and decrease in TBARS with concomitant restoration of the membrane stabilizing enzymes Na+ K+ ATPase, Ca2+ ATPase and Mg2+ ATPase was observed. The results, concluded that SK possess significant stone dissolving property through its diuretic, antioxidant and membrane stabilizing property