期刊
CHROMOSOME RESEARCH
卷 26, 期 3, 页码 115-138出版社
SPRINGER
DOI: 10.1007/s10577-018-9582-3
关键词
satellite; centromere; kinetochore; variation; transcription; noncoding RNA; repetitive DNA; epiallele
资金
- National Science Foundation [DGE-1644868]
- National Institutes of Health [R01 GM124041]
Repetitive DNA, formerly referred to by the misnomer junk DNA, comprises a majority of the human genome. One class of this DNA, alpha satellite, comprises up to 10% of the genome. Alpha satellite is enriched at all human centromere regions and is competent for de novo centromere assembly. Because of thehighly repetitive nature ofalpha satellite, ithas been difficult to achieve genome assemblies at centromeres using traditional next-generation sequencing approaches, and thus, centromeres represent gaps in the current human genome assembly. Moreover, alpha satellite DNA is transcribed into repetitive noncoding RNA and contributes to a large portion of the transcriptome. Recent efforts to characterize these transcripts and their function have uncovered pivotal roles for satellite RNA in genome stability, including silencing selfish DNA elements and recruiting centromere and kinetochore proteins. This review will describe the genomic and epigenetic features of alpha satellite DNA, discuss recent findings of noncoding transcripts produced from distinct alpha satellite arrays, and address current progress in the functional understanding of this oft-neglected repetitive sequence. We will discuss unique challenges of studying human satellite DNAs and RNAs and point toward new technologies that will continue to advance our understanding of this largely untapped portion of the genome.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据