期刊
CHIRALITY
卷 30, 期 3, 页码 227-237出版社
WILEY
DOI: 10.1002/chir.22795
关键词
flow; LD; Couette flow; DNA; FDLD; fluorescence LD; fluorophores; LD
资金
- Biotechnology and Biological Sciences Research Council [BB/F011199/1]
- Marie Curie Initial Training Network [CAS-IDP]
- Engineering and Physical Sciences Research Council [EP/F500378/1]
- Biotechnology and Biological Sciences Research Council [BB/F011199/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1091431, EP/K007394/1] Funding Source: researchfish
- BBSRC [BB/F011199/1] Funding Source: UKRI
- EPSRC [EP/K007394/1] Funding Source: UKRI
Fluorescence detection typically enhances sensitivity and selectivity for fluorescent analytes. The potential for combining fluorescence detection with flow orientation of the sample in the normal configuration of linear dichroism experiments is explored in this work by measuring the fluorescence emitted from flow-orientated DNA-bound ligands and M13 bacteriophage. Data for ethidium bromide, Hoechst 33258, and 4,6-diamidino-2-phenyindole are presented. The theoretical basis of the technique is also presented for instruments running in both the fixed direct-current mode, which is the normal operation mode of circular dichroism spectropolarimeters, and also in fixed high-tension voltage mode. The role of the stray light reaching the detector that results in a spectral shape in fixed direct current mode that resembles the shape of a linear dichroism spectrum, rather than the expected reduced linear dichroism, is also explored.
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