Effects of salinity build-up on the performance and microbial community of partial-denitrification granular sludge with high nitrite accumulation

High inorganic salts inevitably impose a toxic impact on biological treatment processes. In this study, the effect of salinity on the performance and microbial community structures of partial-denitrification (PD) was firstly investigated. Results showed the denitrifying activities of non-domesticated PD sludge were completely inhibited under a temporary high salinity (>= 1.5 wt%). However, after domestication, denitrifying activities maintained above 50% of the maximum with salinity build-up step-by-step from 0.0 wt % to 3.0 wt%. High nitrite production was stably achieved during 120 days with nitrate-to-nitrite transformation ratio around 90%. Further investigation showed extracellular polymeric substances content of PD sludge increased from 184.59 mg gVSS(-1) to 560.64 mg gVSS(-1), accompanied by the elevation of average particle size. This occurred against high salinity as a protective response of PD bacteria. Moreover, Thauera, the functional bacteria of PD system, was still dominant with the relative abundance increasing to 8336% (3.0 wt%) from 51.33% (0.0 wt%). (C) 2018 Elsevier Ltd. All rights reserved.