期刊
CHEMOSPHERE
卷 201, 期 -, 页码 144-158出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2018.02.135
关键词
Oxidative stress; Heat shock proteins; Cytochrome P450; Selection pressure; Mortality; Improved adaptation
资金
- CSIR-IITR
- University Grants Commission, New Delhi [SRF: 20-6/2010(i)EU-IV]
- CSIR [SRF: 19-12/2010(i)EU-IV]
- CSIR-network project [BSC-0103]
Adaptive behaviour of an organism has relevance towards developing better resistance in subsequent generations following xenobiotic exposures. Using a genetically tractable and functional insect model, Drosophila melanogaster, we aimed to examine the resistance of the organism against repeated exposures of benzene, an industrial and environmental-chemical and a class I human carcinogen. While 100 mM benzene exposure to one-day old flies for seven days caused similar to 95% mortality (F0), its exposure to subsequent generations of flies led a significant decrease in mortality with maximum survival (-85%) as evident at F28 generation. While burden of benzene and its toxic metabolites was higher in initial generations, in latter generations (F24-F28), concentrations of less toxic metabolites were higher. In parallel, improved metabolism, less oxidative stress, less induction of hsp60 and hsp70 and higher induction of hsp26 and hsp27 along with increased gene dose ratio of three genes (cyp6g1, mrp1, and cyp12d1) were observed in latter generations of benzene exposed flies with maximum benefit accrued in F28 generation. The resistance developed in flies of F28 generation had a negative impact on reproduction which might be due to a cost against selection. The study demonstrates development of benzene resistance in Drosophila with permanent genetic changes. (C) 2018 Elsevier Ltd. All rights reserved.
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