期刊
FOOD AND BIOPRODUCTS PROCESSING
卷 96, 期 -, 页码 240-244出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.fbp.2015.08.006
关键词
Chiral access; Enzyme immobilization; High pressure; Lipase; Organic solvent; Selectivity; Silica nanoparticle
资金
- Monash University and Commonwealth Scientific and Industrial Research Organisation (CSIRO)
- Deutsche Forschungsgemeinschaft (DFG)
High hydrostatic pressure has been increasingly utilized to improve functions of enzymes, and most of such studies are currently focused on free enzymes in aqueous solution or organic solvent. In this work, Candida rugosa lipase (CRL) was immobilized onto silica nanoparticles and its activity and enantioselectivity in organic solvent were evaluated at high pressures under different water activities. The application of high hydrostatic pressures (50-200 MPa) led to improved activities of immobilized CRL for transesterification of (R)-1-phenylpropan-2-ol with vinyl acetate by 4-6 folds. Additionally the immobilization of CRL resulted in a significant change of selectivities, shifting the enantiomeric excess from the (R)- towards (S)-1-phenylpropan-2-yl acetate product at atmospheric pressure. The application of high pressures led to either enantiomeric excess towards (R)-1-phenylpropan-2-yl or no enantiomeric selectivity, depending on the water activities in the organic solvent and the level of pressures. The interesting behaviour of immobilized CRL under high pressures offers new opportunities to modulate enzyme functions through combination of high pressures and enzyme immobilization. Crown Copyright (C) 2015 Published by Elsevier B.V. on behalf of The Institution of Chemical Engineers. All rights reserved.
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