期刊
JOURNAL OF BREATH RESEARCH
卷 9, 期 4, 页码 -出版社
IOP PUBLISHING LTD
DOI: 10.1088/1752-7155/9/4/047103
关键词
exhaled breath; gas chromatography-mass spectrometry; hypoxia; biomarkers
资金
- UES Inc. under United States Air Force [FA8650-14-D-6516]
Pilots have reported experiencing in-flight hypoxic-like symptoms since the inception of high-altitude aviation. As a result, the need to monitor pilots, in-flight, for the onset of hypoxic conditions is of great interest to the aviation community. We propose that exhaled breath is an appropriate non-invasive medium for monitoring pilot hypoxic risk through volatile organic compound (VOC) analysis. To identify changes in the exhaled breath VOCs produced during periods of reduced O-2 levels, volunteers were exposed to simulated flight profiles, i.e. sea level for 5 min, O-2 levels found at elevated altitudes for 5 min or placebo and 5 min at 100% O-2 recovery gas, using a modified flight mask interfaced with a reduced O-2 breathing device. During the course of these test events, time series breath samples from the flight mask and pre/post bag samples were collected and analyzed by gas chromatography/mass spectrometry (GC/MS). Seven compounds (pentanal, 4-butyrolactone, 2-pentanone, 2-hexanone, 2-cyclopenten-1-one, 3-methylheptane and 2-heptanone) were found to significantly change in response to hypoxic conditions. Additionally, the isoprene, 2-methyl-1,3- butadiene, was found to increase following the overall exposure profile. This study establishes an experimental means for monitoring changes in VOCs in response to hypoxic conditions, a computational workflow for compound analysis via the Metabolite Differentiation and Discovery Lab and MatLab(C) software and identifies potential volatile organic compound biomarkers of hypoxia exposure.
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